Part:BBa_K2206003:Design

Toehold switch for hsa-miR-27b-3p with GFPmut3b

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 758
  

Design Notes

No terminators are needed as the pSB1C3 plasmid contains terminators.

There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein.

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT